DLC Identificatie van vette oliën met behulp van DLC

Identificatie van vette oliën met DLC

De Pharmacopoeia Europea geeft hiervoor het volgende voorschrift:

2.3.2. IDENTIFICATION OF FATTY OILS BY THIN-LAYER CHROMATOGRAPHY Examine by thin-layer chromatography (2.2.27), using as the coating substance a suitable octadecylsilyl silica gel for high perfomance thin-layer chromatography.
Test solution.
Unless otherwise prescribed, dissolve about 20 mg (one drop) of the fatty oil in 3�ml of methylene chloride R. Reference solution.
Dissolve about 20 mg (one drop) of maize oil R in 3 ml of methylene choride R.
Apply separately to the plate 1 �l of each solution.
Develop twice over a path of 0.5 cm using ether R. Develop twice over a path of 8 cm using a mixture of 20 volumes of methylene chloride R, 40 volumes of glacial acetic acid R and 50 volumes of acetone R.
Allow the plate to dry in air and spray with a 100 g/l solution of phosphomolybdic acid R in alcohol R.
Heat the plate at 120��C for about 3 min and examine in daylight.

The chromatogram obtained typically shows spots comparable to the chromatogram below.

1. Arachis oil
2. Olive oil
3. Sesame oil
4. Maize oil
5. Almond oil
6. Soya-bean oil
7. Sunflower oil
8. Rapeseed oil
9. Rapeseed oil (erucic acid-free)
10. Wheat germ oil

Type chromatogram for the identification of fatty oils Farmaceutische Analyse 5e-jaar |

---

10 september 1999